Given below are two statements:
Statement I: The DNA fragments extracted from
gel electrophoresis can be used in construction of
recombinant DNA.
Statement II: Smaller size DNA fragments are
observed near anode while larger fragments are
found near the wells in an agarose gel.
In the light of the above statements, choose the most
appropriate answer from the options given below:
Options :
A.
Both statement I and statement II are correct
B.
Both statement I and statement II are incorrect
C.
Statement I is correct but statement II is
incorrect
D.
Statement I is incorrect but statement II is
correct
Show Answer
Answer :
Both statement I and statement II are correct
Solution :
- The DNA fragments extracted from the gel
electrophoresis can be used in the
construction of recombinant DNA molecules
by joining them with cloning vectors.
-
Since DNA fragments are negatively charged
molecules, therefore they can be separated
by forcing them to move towards the anode
under an electric field through a
medium/matrix.
-
The DNA fragments separate according to
their size through sieving effect provided by
the agarose gel. Hence, the smaller the
fragment size, the farther it moves.
-
Therefore, smaller fragments will be observed
at the anode whereas the larger DNA
fragments near the wells in agarose gel
