Which of the following is true about bundle sheath cells present in the leaves having Kranz anatomy?

Kranz anatomy is a specialized leaf structure characteristic of C₄ plants, such as maize, sugarcane, and sorghum. This anatomical adaptation is designed to optimize carbon dioxide fixation and minimize photorespiration.

Let us analyze the key features of bundle sheath cells in C₄ leaves with Kranz anatomy:
1. Thick Cell Walls: The cell walls of the bundle sheath cells are thick and impermeable to gases. This prevents the leakage of concentrated carbon dioxide from the bundle sheath cells back into the mesophyll cells.
2. No Intercellular Spaces: The bundle sheath cells are tightly packed around the vascular bundles in a wreath-like arrangement (hence the word "Kranz", which means wreath in German). The lack of intercellular spaces prevents gas exchange with the atmosphere, helping to maintain a high concentration of carbon dioxide internally around RuBisCO.
3. Many Chloroplasts: These cells contain a large number of chloroplasts. In many C₄ plants, these chloroplasts are larger and may lack grana (agranal chloroplasts), specialized for carrying out the Calvin cycle reactions where carbon dioxide is fixed into sugars.

Therefore, the correct statement is that bundle sheath cells have thick walls, no intercellular spaces, and many chloroplasts.