This bioassay is used to test the presence of gibberellins
Correct Answer :
dwarf pea elongation technique
Solution :
The correct option is "dwarf pea elongation technique".
Detailed Explanation:
Gibberellins are a group of plant hormones (phytohormones) that play a critical role in regulating various developmental processes, most notably stem elongation, germination, dormancy, flowering, and leaf and fruit senescence.
A bioassay is a biological test used to determine the concentration or activity of a substance (like a hormone) by measuring its effect on a living organism or its parts. For gibberellins, several bioassays are commonly used based on their physiological effects:
1. Dwarf Pea Elongation Technique: Genetic dwarfism in certain plants, such as dwarf pea (Pisum sativum) or dwarf maize, is typically caused by a lack of endogenous active gibberellins. When exogenous gibberellic acid (GA) is applied to these dwarf plants, it overcomes the genetic dwarfism and stimulates rapid stem elongation, making them grow to normal heights. The amount of elongation is proportional to the concentration of gibberellin applied. Therefore, this dwarf pea elongation technique serves as a classic and highly sensitive bioassay for testing the presence and concentration of gibberellins.
2. Other Options Analysis:
• The excised radish cotyledon enlargement test and the chlorophyll preservation test are classic bioassays used for cytokinins (which promote cell division, cotyledon expansion, and delay senescence/chlorophyll degradation).
• The rice seedling growth inhibition test is typically associated with growth inhibitors or hormones like abscisic acid (ABA) rather than the growth-promoting gibberellins.
Hence, the dwarf pea elongation technique is specifically used to test for the presence of gibberellins.
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