Plasmid pBR322 has PstI restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

Options :

1. it will not be able to confer ampicillin resistance to the host cell.

2. the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.

3. it will lead to lysis of host cell.

4. it will be able to produce a novel protein with dual ability.