Identify the correct statement.

The correct statement is: RNA polymerase binds with Rho factor to terminate the process of transcription in bacteria.

Let us analyze each of the given options step-by-step to understand why this statement is correct and why the others are incorrect:

1. Capping of hnRNA:
During post-transcriptional modification in eukaryotes, precursor messenger RNA (hnRNA) undergoes capping and tailing. In capping, an unusual nucleotide, methyl guanosine triphosphate (7-methylguanosine), is added to the 5' end of the hnRNA, not the 3' end. The 3' end is instead modified by the addition of a poly-A tail (polyadenylation). Therefore, this statement is incorrect.

2. Transcription Termination in Bacteria:
In bacteria, transcription termination occurs through two primary mechanisms: Rho-independent (intrinsic) and Rho-dependent termination. In Rho-dependent termination, a protein factor called the Rho (ρ) factor binds to the growing RNA chain, tracks along it behind the RNA polymerase, and interacts with the RNA polymerase to destabilize the transcription complex. This leads to the release of the newly synthesized RNA molecule and the dissociation of RNA polymerase from the DNA template. Therefore, this statement is correct.

3. Template vs. Coding Strand:
In a transcription unit, the two strands of DNA play different roles:
- The template strand (oriented in the 3' to 5' direction) acts as the template for transcription and is actually copied by RNA polymerase to form mRNA.
- The coding strand (oriented in the 5' to 3' direction) has the same sequence as the newly synthesized mRNA (except that thymine in DNA is replaced by uracil in RNA) and is not copied. Therefore, this statement is incorrect.

4. Split Gene Arrangement:
A split gene arrangement refers to the presence of both coding regions (exons) and non-coding intervening sequences (introns) within a gene. This split gene organization is a characteristic feature of eukaryotes. Prokaryotic genes are typically continuous and do not contain introns. Therefore, this statement is incorrect.