Explain the three steps carried out in the formation of recombinant DNA using the enzyme EcoRI.

Answer :

Eco R1 cuts vector DNA , foreign DNA/ gene of interest , at pallindromic site /
3'CTTAAG 5'
5'GAATTC3' (between bases G & A only), sticky end (over hanging stretch of bases) formed at each strand, Joining of sticky ends from DNA fragments by enzyme DNA Ligase, Recombinant DNA(rDNA) is formed