Question Details

DNA fingerprinting recognizes the differences in

Options

A

satellite DNA

B

bulk DNA

C

Repetitive DNA

D

both (a) and (c)

Correct Answer :

both (a) and (c)

Solution :

The correct option is both (a) and (c).

DNA Fingerprinting is a molecular biology technique used to identify and compare individuals based on the unique patterns in their DNA. The basis of this technique lies in identifying variations in specific regions of the genomic sequence.

Here is a detailed step-by-step breakdown of why satellite DNA and repetitive DNA are key to this process:
1. Repetitive DNA: A major portion of the human genome consists of sequences where a small stretch of DNA is repeated many times. These sequences are referred to as repetitive DNA. Because these repetitions generally do not code for proteins, they accumulate mutations over generations without affecting survival, resulting in high levels of variation (polymorphism) between individuals.
2. Satellite DNA: When genomic DNA is separated using density gradient centrifugation, the bulk genomic DNA forms a major peak, while the repetitive DNA sequences separate out as smaller, distinct peaks known as satellite DNA. Depending on the base composition (A-T rich or G-C rich), length of the segment, and the number of repetitive units, satellite DNA is further classified into mini-satellites and micro-satellites.
3. Variable Number Tandem Repeats (VNTRs): Mini-satellites, or VNTRs, show a very high degree of polymorphism that is unique to each individual (except monozygotic twins). DNA fingerprinting utilizes specific probes to identify these VNTR regions, generating a unique banding pattern for every person.

Since satellite DNA is a type of repetitive DNA, and both represent the polymorphic regions analyzed to distinguish individuals, DNA fingerprinting recognizes the differences in both of these types of DNA.

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