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NEET (UG)-2025 Zoology code-45 Question Paper with Solutions

# Q1 of 45

Given below are two statements:


Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below:

Options
A.

Both statement I and statement II are correct

B.

Both statement I and statement II are incorrect

C.

Statement I is correct but statement II is incorrect

D.

Statement I is incorrect but statement II is correct

Show Answer
Correct Answer

Both statement I and statement II are correct

Solution

  • The DNA fragments extracted from the gel electrophoresis can be used in the construction of recombinant DNA molecules by joining them with cloning vectors.
  • Since DNA fragments are negatively charged molecules, therefore  they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.
  • The DNA fragments separate according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves. 
  • Therefore, smaller fragments will be observed at the anode whereas the larger DNA fragments near the wells in agarose gel

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